Sterile Technique Guide

Sterile Technique Guide

For informational and educational purposes only. Not medical advice. Consult a licensed healthcare provider.

1. Workspace

Clean, flat surface away from drafts. Wipe with 70% isopropyl alcohol. Air-dry completely. Lay out all supplies before beginning.

2. Hand Hygiene

Wash 20+ seconds with soap. Dry with lint-free towel.

3. Vial Prep

Remove flip-top cap. Keep metal crimp seal. Swab stopper with alcohol pad (center outward). Air-dry 30 seconds.

4. Drawing

Attach 18G needle (luer-lock). Draw air equal to desired volume. Insert through stopper center. Inject air. Invert vial. Draw fluid. Tap for bubbles. Expel air. Withdraw.

5. Site Prep

Swab area center outward. Air-dry completely.

6. Disposal

Sharps go directly into container. Never recap. Never bend or break.

7. Storage

Sealed: room temp (15-25C), away from light. Punctured: use within 28 days. Keep unused supplies sealed.